Protein Accession NumberNP_000369.3, NP_077742.2, NP_077743.2, NP_077744.3
LocationWT1 peptide corresponds to 15 amino acids of WT1.
PreparationWT1 peptide is supplied as 100μg of dried peptide. When the peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml.
ApplicationWT1 peptide is used for blocking the activity of WT1 antibody (catalog number 46-601). The WT1 antibody ships at 0.5mg/ml and the WT1 peptide ships as a 100μl pellet. When peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
StorageStore at -20˚C. Minimize freezing and thawing.