Protein Accession Number
NP_003378.3, NP_001070737.1Location
WIPF1 peptide corresponds to 15 amino acids of WIPF1.Preparation
WIPF1 peptide is supplied as 100μg of dried peptide. When the peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml.Application
WIPF1 peptide is used for blocking the activity of WIPF1 antibody (catalog number 46-596). The WIPF1 antibody ships at 0.5mg/ml and the WIPF1 peptide ships as a 100μl pellet. When peptide is reconstituted in 200μl water, the concentration will be 0.5mg/ml, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.Buffer
Dried peptide.Storage
Store at -20˚C. Minimize freezing and thawing.
