TRIM29 Antibody | catalog# 42-505
Background
Description
Western blot analysis of TRIM29 in Peripheral Blood Lymphocytes lysate (35 μg protein in RIPA buffer) using TRIM29 antibody (1 μg/mL).
Immunohistochemistry staining of TRIM29 in paraffin embedded human skin using TRIM29 Antibody at 3.8 μg/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Specifications
Additional Names
TRIM29, tripartite motif-containing 29, tripartite motif protein TRIM29, FLJ36085, ATDC, ataxia-telangiectasia group D-associated proteinTested Application
E, WBDisclaimer
This product is for research use only.Protein Accession Number
NP_036233.2Positive Control
- Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate]
Storage
Aliquot and store at -20˚C. Minimize freezing and thawing.Blocking Peptide
Cat. No. 42-505P - TRIM29 PeptideBuffer
TRIM29 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.Application
ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 70 kDa band observed in human peripheral blood mononucleocytes lysates (calculated MW of 65.8 kDa according to NP_036233.2). Recommended concentration: 0.3-1 μg/mL. an additional band of unknown identity was also consistently observed at 37 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human skin shows staining of subnuclear structures and cytoplasm in keratinocytes. Recommended concentration, 1-2 μg/mL.Preparation
Physical State: LiquidConcentration: 500 μg/mL
Immunogen
TRIM29 antibody was raised against a 12 amino acid synthetic peptide near the internal region of TRIM29.Reactivity
Tested Species Reactivity: HumanExpected species reactivity: Human, Murine, Rattus
Host
TRIM29 antibody was raised in goat.Please use anti-goat secondary antibodies.