TGM5 Antibody | catalog# 6537
Background
Transglutaminases (TGM) are a family of structurally and functionally related Ca2+-dependent enzymes (TGases) that stabilize protein assemblies through the formation of gamma-glutamyl-epsilon lysine crosslinks . TGases influence numerous biological processes, including blood coagulation, cell differentiation, fertilization and apoptosis . TGM5 belongs to the transglutaminase superfamily and catalyzes the cross-linking of proteins between glutamine and lysine residues, often resulting in stabilization of protein assemblies . TGM5 is also expressed in the human hair follicle . Defects in TGM5 are associated with acral peeling skin syndrome .
Description
Western blot analysis of TGM5 in rat heart tissue lysate with TGM5 antibody at (A) 1 and (B) 2 μg/mL.
Specifications
Protein Accession Number
NP_963925Purification
Affinity chromatography purified via peptide columnClonality/Clone
This is a polyclonal antibody.Host
TGM5 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Specificity
TGM5 antibody is predicted to not cross-react with other TGase protein family members. Multiple isoforms of TGM5 are known to exist.Immunogen
TGM5 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TGM5.Application
TGM5 antibody can be used for detection of TGM5 by Western blot at 1 - 2 μg/mL.Tested Application
E, WBBuffer
Antibody is supplied in PBS containing 0.02% sodium azide.Blocking Peptide
Cat. No. 6537 - TGM5 PeptideStorage
TGM5 Antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.Reference
- Ueki S, et al. Dual functions of transglutaminase in novel cell adhesion. J. Cell Sci. 1996; 109:2727-35.
- Candi E, Oddi S, Terrinoni A, et al. Transglutaminase 5 cross-links loricrin, involicrin, and small proline-rich proteins in vitro. J. Biol. Chem. 2001; 276:35014-23.
- Thibaut S, Candi E, Pietroni V, et al. Transglutaminase 5 expression in human hair follicle. J. Invest. Dermatol. 2005; 125:581-5
- Cassidy AJ, van Steensel MA, Steijlen PM, et al. A homozygous missense mutation in TGM5 abolishes epidermal transglutaminase 5 activity and causes acral peeling skin syndrome. Am. J. Hum. Genet. 2005; 77:909-17. ]
Positive Control
- Cat. No. 1461 - Rat Heart Tissue Lysate]