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TCTN3 Antibody | catalog# 5837

Background

TCTN3 Antibody: TCTN3 is a member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. While the expression pattern and function of TCTN3 is not known, it is 58% similar to TCTN1. TCTN1 is expressed during embryonic development in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

Description
TCTN3 Antibody
Western blot analysis of TCTN3 in HeLa cell lysate with TCTN3 antibody at (A) 1 and (B) 2μg/mL.
TCTN3 Antibody
Immunocytochemistry of TCTN3 in HeLa cells with TCTN3 antibody at 4 μg/mL.
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Specifications

Additional Names
TCTN3 Antibody: Tectonic family member 3, TECT3
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
TCTN3 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human TCTN3.
Isotype
IgG
Clonality/Clone
This is a polyclonal TCTN3 Antibody.
Conjugate
None
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Purification
TCTN3 Antibody is affinity chromatography purified via peptide column.
Host
TCTN3 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
TCTN3 antibody can be used for detection of TCTN3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 4 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
TCTN3 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5837P - TCTN3 Peptide
Storage
TCTN3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1220 - SK-N-SH Cell Lysate]
Species Reactivity
H, M, R
Protein GI Number
221136774
Protein Accession Number
NP_056446
Reference
  1. Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7.]