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TCTN1 Antibody | catalog# 5833

Background

TCTN1 Antibody: TCTN1 is the founding member of the Tectonic protein family, a group of evolutionarily conserved secreted and transmembrane proteins that regulate the Hedgehog (Hh)-mediated patterning of the neural tube. During embryonic development, TCTN1 is expressed in regions that participate in Hh signaling, beginning in the gastrulation stages in the ventral node. Mice expressing mutant TCTN1 die between E13.5 and E16.5 and display holoprosencephaly, a defect associated with reduced Hh signaling, indicating the role of TCTN1 as an Hh activator. At later stages in development, TCTN1 is thought to also act as a repressor on the Hh pathway in the anterior and posterior neural tube.

Description
TCTN1 Antibody
Western blot analysis of TCTN1 in mouse kidney tissue lysate with TCTN1 antibody at (A) 1 and (B) 2μg/mL.
TCTN1 Antibody
Immunohistochemistry of TCTN1 in human kidney tissue with TCTN1 antibody at 5 μg/mL.
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Specifications

Additional Names
TCTN1 Antibody: Tectonic family member 1, TECT1
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
TCTN1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human TCTN1.
Isotype
IgG
Clonality/Clone
This is a polyclonal TCTN1 Antibody.
Conjugate
None
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Purification
TCTN1 Antibody is affinity chromatography purified via peptide column.
Host
TCTN1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
TCTN1 antibody can be used for detection of TCTN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
TCTN1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5833P - TCTN1 Peptide
Storage
TCTN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1405 - Human Kidney Tissue Lysate]
Species Reactivity
H, M
Protein GI Number
130978359
Protein Accession Number
NP_001076007
Reference
  1. Reiter JF and Skarnes WC. Tectonic, a novel regulator of the Hedgehog pathwy required for both activation and inhibition. Genes Dev.2006; 20:22-7.]