TAF10 Antibody | catalog# 27-340
Background
TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. TAF10 is one of the small subunits of TFIID that is associated with a subset of TFIID complexes. It is required for transcriptional activation by the estrogen receptor, for progression through the cell cycle, and may also be required for certain cellular differentiation programs.
Description
Western blot analysis of TAF10 in Jurkat Cell Lysate using TAF10 antibody at 0.5 μg/ml (12% gel). Marker in lane A.
Specifications
Additional Names
TAF10Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
TAF10 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human TAF10.Application
TAF10 antibody can be used for detection of TAF10 by ELISA at 1:1562500. TAF10 antibody can be used for detection of TAF10 by western blot at 0.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store TAF10 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, M, DProtein Accession Number
NP_006275Molecular Weight
22 kDaReference
- Soutoglou, E., et al., (2005) Mol. Cell. Biol. 25 (10), 4092-4104.]