Skeletal Muscle Cytoplasmic Diabetic Disease Lysate

Skeletal Muscle Cytoplasmic Diabetic Disease Lysate | catalog# XBL-10854

Background

human skeletal muscle tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human skeletal muscle tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated Skeletal Muscle tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Skeletal Muscle tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

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Specifications

Tissue Type

Skeletal Muscle

Diagnosis

Diabetic Disease

Source

Human Skeletal Muscle tissue

Application

Skeletal Muscle cytoplasmic protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.

Tested Application

Storage

Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.