Location18 amino acids near the carboxy terminus of human SAE2.
ApplicationSAE2 peptide is used for blocking the activity of SAE2 antibody (catalog number 5751). It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37˚C.
BufferPBS containing 0.02% sodium azide
StorageSAE2 peptide is supplied as 50 μg at 200 μg/ml in PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide. Store at -20˚C stable for one year.
- Desterro JM, Rodriguez MS, Kemp GD, et al. Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1. J. Biol. Chem. 1999; 274:10618-24.]