Location
S1PR2 peptide was raised against a synthetic peptide corresponding to the internal region of S1PR2.Preparation
S1PR2 peptide is supplied as 100 μg of dried peptide. When the peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL.Application
S1PR2 peptide is used for blocking the activity of S1PR2 antibody (catalog number 42-636). The S1PR2 antibody ships at 0.5 mg/mL and the S1PR2 peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μL water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.Buffer
Dried peptideStorage
Store at -20˚C. When reconstituted minimize freezing and thawing.Protein Accession Number
NP_004221.3Disclaimer
This product is for research use only.
