Redi-Pro Antibody Biotinylation Kit

Redi-Pro Antibody Biotinylation Kit | catalog# PSI-4001

Background

The Redi-Pro(TM) Antibody Biotinylation Kit offers the complete solution to the quantitative biotinylation of 100μg of antibody. The kit incorporates traceable biotin and allows for direct spectrophotometric verification of biotin incorporation with a simple A280 and A354 measurement to determine the number of biotins per antibody molecule.

Product Resources

Antibody Biotinylation Protocol (PDF)
Biotin MSR Calculator (Excel File)
Detailed Instruction Booklet and Troubleshooting (PDF)

Description

Sulfo-Redi-Pro™ Biotin

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Specifications

Kit Contents

8.0μg Sulfo-Redi-Pro™ Biotin
1.5mL 1x Modification Buffer (pH 7.4)
1.5mL 1x PBS (pH 7.2)
4 1.5mL Collection tubes
2 0.5mL Zeba™ Spin Columns
100μg Biotinylated IgG Control
100μL 1M Tris-HCL (pH 8.9)

Application

Incorporation of biotin into amine-containing biomolecules. Linker possesses a chromophore allowing for direct spectroscopic quantification of biotin incorporation.

Tested Application

Biotinylation

Storage

All Redi-Pro™ Biotin One-Shoot Kit components should be stored at room temperature.

Species Reactivity

n/a