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Raji Nuclear Lysate | catalog# XBL-10437

Background

Raji (Human lymphoma; B lymphoma) cell nuclear protein lysate was prepared by isolating the nuclear protein from whole tissue homogenates using a proprietary technique. TheRaji (Human lymphoma; B lymphoma) cell line cell line was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The nuclear protein is provided in a buffer including HEPES (pH7.9), glycerol, NaCl, MgCl2, EDTA, and a cocktail of protease inhibitors. For quality control purposes, the isolated Raji (Human lymphoma; B lymphoma) cell nuclear protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated Raji (Human lymphoma; B lymphoma) cell nuclear protein is then Western analyzed by histone antibody to confirm that the expression level is consistent with each lot.

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Specifications

Tissue Type
Raji (Human Lymphoma; B Lymphoma)
Diagnosis
Tumor
Tested Application
WB
Source
Raji (Human Lymphoma; B Lymphoma) cell line
Application
Raji (Lymphoma; B Lymphoma) cell line nuclear protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Human