Rabphilin Antibody

Rabphilin Antibody | catalog# 50-236

Background

Rabphilin-3A is a peripheral membrane protein that binds Ca2+ and phospholipids and is attached to synaptic vesicle membranes (Geppert et al., 1994;Li et al., 1994). The expression of rabphilin 3A is reduced in an animal model of Huntington's disease(Smith et al., 2005). Rabphilin can be phosphorylated at Ser234 by both CAM kinase II and PKA and this phosphorylation has been suggested to regulate neuronal activity during development in a synapse-specific manner (Fykse et al., 1995;Foletti and Scheller, 2001).

Description

Western blot of rat brain lysate showing specific immunolabeling of the ~82k rabphilin 3A phosphorylated at Ser234.

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Specifications

Clonality/Clone

This is a polyclonal antibody.

Purification

Affinity Purified

Host

Rabphilin antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.

Immunogen

Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser234 of rabphilin 3A.

Application

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with mouse based on the fact that this species has 100% homology with the amino acid sequence used as antigen.

Tested Application

Buffer

Rabphilin antibody is supplied as affinity purified polyclonal antibody, 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.

Storage

Rabphilin antibody can be stored at -20˚C and is stable at -20˚C for at least 1 year.

Positive Control

Cat. No. 1463 - Rat Brain Lysate]

Species Reactivity

M, R

Reference

Foletti DL, Scheller RH (2001) Developmental regulation and specific brain distribution of phosphorabphilin. J Neurosci 21:5461-5472.
Fykse EM, Li C, Sudhof TC (1995) Phosphorylation of rabphilin-3A by Ca2+/calmodulin- and cAMP-dependent protein kinases in vitro. J Neurosci 15:2385-2395.
Geppert M, Bolshakov VY, Siegelbaum SA, Takei K, De Camilli P, Hammer RE, Südhof TC (1994) The role of Rab3A in neurotransmitter release. Nature (Lond ) 369:493-497.]