FormulationRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
Clonality/CloneThis is a polyclonal antibody.
SpecificityHuman (71% homologous) p53. Mouse and rat (100% homologous) p53 have not been tested, but are expected to react.
SourceRabbit p53 antibody was produced against a chemically synthesized phosphopeptide derived from a region of mouse p53 that contains serine 392. The sequence is conserved in rat.
PurificationRabbit p53 antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p53. The final product is generated by affinity chromatography using a p53-derived peptide that is phosphorylated at serine 392.
HostRabbit p53 [pS392] antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ApplicationFor Western blotting applications, we recommend using the antibody at a 1:1000 dilution.
BufferRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
StorageStore at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For shipment or short-term storage (up to one week), 2 - 8˚C is sufficient.
- Fogarty, M.P., et al. (2003) A role for c-Jun N-terminal kinase 1 (JNK1), but not JNK2, in the beta-amyloid-mediated stabilization of protein p53 and induction of the apoptotic cascade in cultured cortical neurons. Biochem. J. 371(Pt 3):789-798.
- McKendrick, L., et al. (1999) Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable. Mol. Cell. Biochem. 191(1-2):187-199.
- Kapoor, M. and G. Lozano (1998) Functional activation of p53 via phosphorylation following DNA damage by UV but not gamma radiation. Proc. Nat'l. Acad. Sci. USA 95(6):2834-2837.
- Hao, M., et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J. Biol. Chem. 271(46): 29380-29385.]