Rabbit p53 [pS392] Antibody | catalog# XBP-4240
Background
p53 is a nuclear adapter protein whose multiple functions include cell cycle regulation and suppression of tumor cell growth. Normal p53 function has been shown to be extremely vital as almost half of all human cancers exhibit a defect in p53. Phosphorylation of serine 392 by PKR or Casein Kinase II (CK2) is required for site-specific DNA binding, strand renaturation, down-regulation of transcription and proliferation, leading to the induction of apoptosis and causing cell cycle arrest.
Description
![Rabbit p53 [pS392] Antibody](./image/product/04/17/XBP-4240.gif)
HEK 293 extracts were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was either untreated (lanes 1-4) or treated with 4000 units/mL lambda (δ) phosphatase at 37˚C for 1 hour (lane 5), blocked with a 5% BSA-TBST buffer overnight at 4˚C, and then incubated with the p53 [pS392] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (lane 1), the non-phosphopeptide corresponding to the phosphopeptide immunogen (lane 2), a generic phosphoserine-containing peptide (lane 3), or the phosphopeptide immunogen (lane 5). After washing, the membrane was incubated with goat F(ab')2 anti-rabbit IgG HRP conjugate and signals were detected using the Pierce SuperSignal™ method.
Specifications
Formulation
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Clonality/Clone
This is a polyclonal antibody.Specificity
Human (71% homologous) p53. Mouse and rat (100% homologous) p53 have not been tested, but are expected to react.Source
Rabbit p53 antibody was produced against a chemically synthesized phosphopeptide derived from a region of mouse p53 that contains serine 392. The sequence is conserved in rat.Purification
Rabbit p53 antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p53. The final product is generated by affinity chromatography using a p53-derived peptide that is phosphorylated at serine 392.Host
Rabbit p53 [pS392] antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Application
For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.Tested Application
WBBuffer
Rabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.Storage
Store at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For shipment or short-term storage (up to one week), 2 - 8˚C is sufficient.Positive Control
- HEK 293 extracts.]
Species Reactivity
M, RReference
- Fogarty, M.P., et al. (2003) A role for c-Jun N-terminal kinase 1 (JNK1), but not JNK2, in the beta-amyloid-mediated stabilization of protein p53 and induction of the apoptotic cascade in cultured cortical neurons. Biochem. J. 371(Pt 3):789-798.
- McKendrick, L., et al. (1999) Protein kinase CK2-dependent regulation of p53 function: evidence that the phosphorylation status of the serine 386 (CK2) site of p53 is constitutive and stable. Mol. Cell. Biochem. 191(1-2):187-199.
- Kapoor, M. and G. Lozano (1998) Functional activation of p53 via phosphorylation following DNA damage by UV but not gamma radiation. Proc. Nat'l. Acad. Sci. USA 95(6):2834-2837.
- Hao, M., et al. (1996) Mutation of phosphoserine 389 affects p53 function in vivo. J. Biol. Chem. 271(46): 29380-29385.]