ProSci Incorporated

Antibodies For All Your Research

Production and Services

POLYCLONAL ANTIBODY PRODUCTION

Polyclonal Antibodies are a mixture of immunoglobulins that recognize different epitopes on a specific antigen. ProSci produces polyclonal antibodies in rabbits, chickens, or goats. The antibodies are isolated from the serum or from the yolks of the chicken eggs. Serum can be used directly or further purified, but yolks are further purified prior to use to remove the lipids that can interfere in assays.

ProSci Incorporated owns and operates a USDA licensed and NIH approved animal facility in San Diego County, California. We offer a standard 8-week production protocol that balances fast turnaround time with quality. We offer packages for client-provided antigens, single-peptide projects, peptide cocktail projects, and phospho-peptide projects. An overview of our standard packages includes:

Package 1: Antibody Production using Client-Provided Antigen:

  • 8-week immunization protocol in rabbits, chickens, or goats
    • Pre-immune serum
    • 4 immunizations using client-provided antigen
    • 3 production bleeds per animal

Package 2: Antibody Production using peptide synthesized by ProSci:

  • Synthesis of peptide up to 15 residues (5mg for customer)
  • Conjugation of peptide to carrier protein
  • Immunization of conjugate into rabbits, chickens, or goats on 8 week protocol
  • ELISA on Bleed #1 to verify titers using unconjugated peptide

Package 3: Antibody Production using peptide synthesized by ProSci:

  • Synthesis of peptide up to 15 residues (5mg for customer)
  • Conjugation of peptide to carrier protein
  • Immunization of conjugate into rabbits, chickens, or goats on 8 week protocol
  • ELISA on Bleed #1 to verify titers using unconjugated peptide
  • Immuno-Affinity Purification of 25mL serum on Bleed #3 (includes re-usable column)

Package 4: Antibody Production using peptide synthesized by ProSci:

  • Synthesis of peptide up to 15 residues (5mg for customer)
  • Conjugation of peptide to carrier protein
  • Immunization of conjugate into rabbits, chickens, or goats on 8 week protocol
  • ELISA on Bleed #1 to verify titers using unconjugated peptide
  • Immuno-Affinity Purification of 150-200mL serum(includes re-usable column)

Upon completion of the initial 8-week protocol there are several options to select from:

Termination

  • Rabbits and chickens are euthanized and goats are retired without any additional services

Monthly Extension

  • 1 immunization
  • 2 production bleeds

Exsanguination (Rabbits only)

  • Series of 2 bleeds about 4 days apart that yield ~60mL serum

Peptide Cocktail Antibody Production packages include all of the services in Package 2 and Package 3 respectively, but include the synthesis, conjugation, and immunization of your choice of 2 or 3 peptides into one set of animals. This is an option that increases the likelihood of a peptide-based antibody working for multiple applications, as it doubles or triples the available number of epitopes for the animals' immune systems to respond against. It also helps to reduce overall cost, as it minimizes the number of animals needed for a particular project.

Phospho-Specific Antibody Production packages include all of the services in Package 2, 3, and 4 respectively, but include the synthesis, conjugation, and immunization of phospho peptides into a set of rabbits. Packages 2PN, 3PN, and 4PN also offer the synthesis of the corresponding non-phospho peptide sequence, used for isolating both the phospho and non-phospho antibodies for your target protein.