PAX5 Antibody | catalog# 28-164
Background
The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.
Description
Western blot analysis of PAX5 in Jurkat Cell Lysate using PAX5 antibody at 2 μg/ml (12% gel).
Specifications
Additional Names
PAX5Disclaimer
This product is for research use only.Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
PAX5 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX5.Application
PAX5 antibody can be used for detection of PAX5 by ELISA at 1:312500. PAX5 antibody can be used for detection of PAX5 by western blot at 2.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WB, IHCBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, M, R, D, ZFProtein Accession Number
NP_057953Molecular Weight
42 kDaReference
- Robichaud, G.A., et al., (2004) J. Biol. Chem. 279 (48), 49956-49963.]