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OMI Antibody | catalog# 3319

Background

OMI Antibody: Inhibitor of apoptosis proteins (IAPs) were initially identified in baculoviruses as proteins that inhibit apoptosis of the host cells to allow time for viral replication. Cellular homologues containing at least one baculoviral IAP repeat (BIR) motif essential for their anti-apoptosis activity have been identified in yeasts and higher organisms and often act by binding and inhibiting processed caspases. The activity of these proteins can be modulated by the expression of proteins such as Smac/DIABLO and XAF-1 which displace or prevent the binding of caspases by IAPs. Recently, a mitochondrial serine protease termed Omi/HtrA2 has been found to bind IAPs. Similar to Smac, Omi possesses a conserved IAP-binding motif, but acts to cleave IAPs to irreversibly inactivate IAPs and promote apoptosis.

Description
OMI Antibody
Western blot analysis of OMI in human colon cell lysates with OMI antibody at (A) 0.5 and (B) 1 μg/mL.
OMI Antibody
Immunohistochemistry of OMI in human colon tissue with OMI antibody at 10 μg/mL.
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Specifications

Additional Names
OMI Antibody: OMI, Htr2A
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal OMI Antibody.
Purification
OMI Antibody is Ion exchange chromatography purified.
Host
OMI antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
OMI antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human OMI.
Application
OMI antibody can be used for detection of OMI by Western blot at 0.5 to 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
OMI Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3319P - OMI Peptide
Storage
OMI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1320 - Human Colon Tissue Lysate]
Species Reactivity
H
Protein GI Number
5870865
Protein Accession Number
AAB94569
Reference
  1. Crook NE, Clem RJ, and Miller LK. An apoptosis inhibiting baculovirus gene with a zinc finger like motif. J. Virol. 1993; 67:2168-2174.
  2. Liston P, Fong WG, and Korneluk RG. The inhibitors of apoptosis: there is more to life than Bcl2. Oncogene 2003; 22:8568-80.
  3. Vaux DL and Silke J. Mammalian mitochondrial IAP binding proteins. Biochem. Biophys. Res. Comm. 2003; 304:499-504.
  4. Suzuki Y, Imai Y, Nakayama H, et al. A serine protease, HtrA2, is released from the mitochondria and interacts with XIAP, inducing cell death. Mol. Cell 2001; 8:613-21.]