NO66 Antibody | catalog# 46-636
Background
Description
Western blot analysis of NO66 in A431 cell lysate (35μg protein in RIPA buffer) using NO66 antibody (0.3μg/mL).
Specifications
Additional Names
C14orf169, chromosome 14 open reading frame 169, FLJ21802, NO66, MAPJDPreparation
Physical State: LiquidConcentration: 500 μg/mL
Immunogen
NO66 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NO66.Reactivity
Tested Species Reactivity: HumanExpected species reactivity: Human, Murine, Rattus
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Source
NO66 antibody was raised against a synthetic peptide of NO66.Purification
NO66 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.Host
NO66 antibody was raised in goat.Please use anti-goat secondary antibodies.
Application
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 70 kDa band observed in lysates of cell line A431 (calculated MW of 71.1 kDa according to NP_078920.2). Recommended concentration: 0.3-1 μg/mL.Tested Application
E, WBBuffer
NO66 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.Blocking Peptide
Cat. No. 46-636P - NO66 PeptideStorage
Aliquot and store at -20˚C. Minimize freezing and thawing.Positive Control
- Cat. No. 1202 - A431 Cell Lysate]
Species Reactivity
HProtein Accession Number
NP_078920.2Reference
- Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS. NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.]