Additional NamesC14orf169, chromosome 14 open reading frame 169, FLJ21802, NO66, MAPJD
PreparationPhysical State: Liquid
Concentration: 500 μg/mL
ImmunogenNO66 antibody was raised against an 11 amino acid synthetic peptide near the internal region of NO66.
ReactivityTested Species Reactivity: Human
Expected species reactivity: Human, Murine, Rattus
DisclaimerThis product is for research use only.
Clonality/CloneThis is a polyclonal antibody.
SourceNO66 antibody was raised against a synthetic peptide of NO66.
PurificationNO66 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HostNO66 antibody was raised in goat.
Please use anti-goat secondary antibodies.
ApplicationELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 70 kDa band observed in lysates of cell line A431 (calculated MW of 71.1 kDa according to NP_078920.2). Recommended concentration: 0.3-1 μg/mL.
BufferNO66 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Blocking PeptideCat. No. 46-636P - NO66 Peptide
StorageAliquot and store at -20˚C. Minimize freezing and thawing.
- Cat. No. 1202 - A431 Cell Lysate]
Protein Accession NumberNP_078920.2
- Eilbracht J, Reichenzeller M, Hergt M, Schnolzer M, Heid H, Stohr M, Franke WW, Schmidt-Zachmann MS. NO66, a highly conserved dual location protein in the nucleolus and in a special type of synchronously replicating chromatin. Mol Biol Cell. 2004 Apr;15(4):1816-32. Epub 2004 Jan 23.]