Additional NamesNCF1, NOXO2, p47phox, neutrophil cytosolic factor 1 (47 kDa, chronic granulomatous disease, autosomal 1), Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)
PreparationPhysical State: Liquid
Concentration: 500 μg/mL
ImmunogenNCF1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of NCF1.
ReactivityTested Species Reactivity: Human
Expected species reactivity: Human, Murine, Rattus, Rabbit, Porcine, Bovine
DisclaimerThis product is for research use only.
Clonality/CloneThis is a polyclonal antibody.
SourceNCF1 antibody was raised against a synthetic peptide near the C-terminus of NCF1.
PurificationNCF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
HostNCF1 antibody was raised in goat.
Please use anti-goat secondary antibodies.
ApplicationELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 47 kDa band seen in U937 cell lysates. Recommended for use at 0.2-2 μg/mL.
BufferNCF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Blocking PeptideCat. No. 45-096P - NCF1 Peptide
StorageAliquot and store at -20˚C. Minimize freezing and thawing.
- Cat. No. 1215 - U937 Cell Lysate]
Protein Accession NumberNP_000256.3
- Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002 Apr 1;363(Pt 1):95-103]