NCF1 Antibody | catalog# 45-096
Background
Description
Western blot analysis of NCF1 in U937 lysate (RIPA buffer, 30μg total protein per lane) using NCF1 antibody (0.2μg/mL).
Specifications
Additional Names
NCF1, NOXO2, p47phox, neutrophil cytosolic factor 1 (47 kDa, chronic granulomatous disease, autosomal 1), Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1)Preparation
Physical State: LiquidConcentration: 500 μg/mL
Immunogen
NCF1 antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of NCF1.Reactivity
Tested Species Reactivity: HumanExpected species reactivity: Human, Murine, Rattus, Rabbit, Porcine, Bovine
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Source
NCF1 antibody was raised against a synthetic peptide near the C-terminus of NCF1.Purification
NCF1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.Host
NCF1 antibody was raised in goat.Please use anti-goat secondary antibodies.
Application
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 47 kDa band seen in U937 cell lysates. Recommended for use at 0.2-2 μg/mL.Tested Application
E, WBBuffer
NCF1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.Blocking Peptide
Cat. No. 45-096P - NCF1 PeptideStorage
Aliquot and store at -20˚C. Minimize freezing and thawing.Positive Control
- Cat. No. 1215 - U937 Cell Lysate]
Species Reactivity
HProtein Accession Number
NP_000256.3Reference
- Davidson-Moncada JK, Lopez-Lluch G, Segal AW, Dekker LV Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils. Biochem J 2002 Apr 1;363(Pt 1):95-103]