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NAT11 Antibody | catalog# 5549

Background

NAT11 Antibody: N-terminal acetylation is one of the most common protein modifications in eukaryotes, occurring on approximately 57% and 84% on yeast and human proteins respectively. There are several N-terminal acetylating enzyme complexes (NatA - NatE). Unlike the other complexes, NatD is composed of a single protein, NAT11, and has recently been described to acetylate the Serine N-termini of histones H2A and H4 in yeast. The role these modifications play is unknown; yeast that do not express NAT11 grow at normal rates and have no observable phenotypes. The role of the human homolog is likewise unknown.

Description
NAT11 Antibody
Western blot analysis of NAT11 in human thymus tissue lysate with NAT11 antibody at (A) 1 and (B) 2 μg/mL.
NAT11 Antibody
Immunohistochemistry of NAT11 in mouse thymus tissue with NAT11 antibody at 5 μg/mL.
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Specifications

Additional Names
NAT11 Antibody: N-acetyltransferase 11, N(alpha)-acetyl transferase 40, NAA40
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
NAT11 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human NAT11.
Isotype
IgG
Clonality/Clone
This is a polyclonal NAT11 Antibody.
Conjugate
None
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Purification
NAT11 Antibody is affinity chromatography purified via peptide column.
Host
NAT11 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
NAT11 antibody can be used for detection of NAT11 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
NAT11 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5549P - NAT11 Peptide
Storage
NAT11 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1314 - Human Thymus Tissue Lysate]
Species Reactivity
H, M
Protein GI Number
189571650
Protein Accession Number
NP_079047
Reference
  1. Arnesen T, Van Damme P, Polevoda B, et al. Proteomics analyses reveal the evolutionary conservation and divergence of N-terminal acetyltransferases from yeast and humans. Proc. Natl. Acad. Sci. USA2009; 106:8157-62.
  2. OK Song, Wang X, Waterborg JH, et al. An Nalpha-acetyl-transferase responsible for acetylation of the N-terminal residues of histones H4 and H2A. J. Biol. Chem.2003; 278:38109-1]