Mouse immunoblots from ProSci are made using mouse cell lines or mouse tissues. ProSci mouse immunoblots are designed for quick and easy protein expression screening of multiple mouse cell lines or mouse tissues. Each mouse immunoblot contains nine lanes, including a pre-stained protein marker and each lane is loaded with 15 μg of total protein. However, our immunoblots are highly customizable. Up to 50 μg of protein can be run on virtually any percentage or gradient percentage gel. Mouse immunoblot membranes can have more than 9 lanes loaded in any order. Our mouse immunoblots are usually transferred to nitrocellulose, but PVDF membranes may also be requested.
ProSci mouse immunoblots can be used with any standard membrane procedure. Our mouse immunoblots can also be reused after stripping off any assay antibodies from the membrane.