MONOCLONAL ANTIBODY DEVELOPMENT AND PRODUCTION

Monoclonal antibodies are secreted by an immortal cell line, typically derived from spleen cells, and fused with a myeloma cell line to make them immortal. They recognize only one epitope on an antigen. Once a single, stable clone is isolated, the antibodies can be made either in vitro or in vivo with ascites or cell culture supernatant.

ProSci can help develop custom monoclonal antibodies and we understand researchers have varying needs for levels of involvement, depending on the target. We have developed monoclonal antibody development packages for different levels of involvement in the project, from researcher screening (you screen positive wells following fusion and during sub-cloning for clones of interest) to ProSci sending Western blot and immunocytochemistry positive final clones. We offer custom monoclonal antibody development packages for both protein and peptide antigens. Our screening options include:

• ProSci sends ELISA positive final clones.
• Researcher screening of positive wells, following fusion.
• Western blot screening: ProSci screening of positives during Phase 2 (Fusion) and Phase 3 (Sub-cloning) for Western blot positive clones.
• ICC screening: ProSci screening of positives during Phase 2 (Fusion) and Phase 3 (Sub-cloning) for immunocytochemistry positive clones
• Western blot & immunocytochemistry screening: ProSci screening of positives during Phase 2 (Fusion) and Phase 3 (Sub-cloning for Western Blot AND immunocytochemistry positive clones.

ProSci's fusion success rate for custom monoclonal antibody development is greater than 95%, using techniques developed in-house. All work is performed by skilled technicians on premises in our San Diego, California laboratories. We can screen against multiple antigens (additional charges apply) to develop the specific clone you need. ProSci has repeatedly proven our abilities to produce hybridomas against difficult targets.

We use 5 BALB/c mice as a production strain for hybridoma development. BALB/c is a very common species used throughout the industry for monoclonal antibody development due to their large spleen to body size ratio. This yields a higher number of splenocytes at fusion.

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