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MECL1 Antibody | catalog# 42-017

Background

Description
MECL1 Antibody
Western blot analysis of MECL1 in human lung lysate (35μg protein in RIPA buffer) using MECL1 antibody (0.3μg/ml).
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Specifications

Additional Names
PSMB10, proteasome (prosome, macropain) subunit, beta type, 10, LMP10, MECL1, MGC1665, beta2i, macropain subunit MECl-1, multicatalytic endopeptidase complex subunit MECl-1, proteasome MECl-1, proteasome beta 10 subunit, proteasome catalytic subunit 2i, proteasome subunit MECL1, proteasome subunit beta 7i
Disclaimer
This product is for research use only.
Preparation
Physical State: Liquid

Concentration: 500 μg/mL
Immunogen
MECL1 antibody was raised against a 12 amino acid synthetic peptide near the internal region of MECL1.
Clonality/Clone
This is a polyclonal antibody.
Positive Control
  • Cat. No. 1302 - Human Lung Tissue Lysate]
Source
MECL1 antibody was raised against a synthetic peptide cooresponding to the internal region of MECL1.
Purification
MECL1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Host
MECL1 antibody was raised in goat.

Please use anti-goat secondary antibodies.
Application
ELISA: Antibody detection limit dilution 1:8000. Western Blot: Approximately 26 kDa band observed in human, liver, lung and lymph node lysates (calculated MW of 28.9 kDa according to NP_002792.1). Recommended concentration: 0.3-1 μg/mL.
Tested Application
E, WB
Buffer
MECL1 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Blocking Peptide
Cat. No. 42-017P - MECL1 Peptide
Storage
Aliquot and store at -20˚C. Minimize freezing and thawing.
Species Reactivity
H
Protein Accession Number
NP_002792.1
Reference
  1. Moschonas A, Kouraki M, Knox PG, Thymiakou E, Kardassis D, Eliopoulos AG. CD40 induces antigen transporter and immunoproteasome gene expression in carcinomas via the coordinated action of NF-kappaB and of NF-kappaB-mediated de novo synthesis of IRF-1. Molecular and Cellular Biology 2008 Oct 28 (20): 6208-22.]