Additional NamesABCD 1, CC chemokine STCP 1, CCL 22, MGC34554, SCYA22, Small inducible cytokine subfamily A Cys Cys member 22, STCP 1, Stimulated T cell chemotactic protein 1
PreparationA sterile filtered antibody solution was lyophilized from PBS, pH 7.2.
Clonality/CloneThis is a polyclonal antibody.
SourceProduced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mMDC. Anti-Murine MDC specific antibody was purified by affinity chromatography and then biotinylated.
HostMDC antibody was raised in rabbit.
Please use rabbit secondary antibodies.
To detect mMDC by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mMDC.
To detect mMDC by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Murine MDC as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant mMDC.
To detect mMDC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMDC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
StorageMDC lyophilized antibody is stable for at least 6 months when kept at -20˚C. The biotinylated antibody should be reconstituted to a concentration of 50 μg/ml with sterile PBS solution containing 0.1% BSA. This solution can be stored at 4˚C for at least one month without detectable loss of activity. Frozen aliquots of this solution are stable for at least 6 months when kept at -20˚C. Avoid more than one freeze-thaw cycle.