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Liver Membrane Liver Cirrhosis Lysate | catalog# XBL-10671

Background

Human liver tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human liver tissue was frozen in liquid nitrogen immediately after excision and then stored at -70˚C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated liver tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated liver tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

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Specifications

Disclaimer
This product is for research use only.
User Note
Ships on Dry Ice
Tissue Type
Liver
Diagnosis
Liver Cirrhosis
Source
Human Liver tissue
Application
Liver membrane protein lysate is for use in Western blotting, 10 μg to 20 μg per lane is recommended for mini gel.
Tested Application
WB
Storage
Store at 2-8˚C for continuous use. For extended storage, freeze working aliquots at -70˚C. Repeated freezing and thawing is not recommended. Under proper storage conditions the shelf life is half a year from the date of receipt.
Species Reactivity
n/a
Species
Human