FormulationRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
Clonality/CloneThis is a polyclonal antibody.
SpecificityHuman Lck. Other species/Src family kinases that have not been tested include: mouse Src (100% homologous with the immunogen), chicken Src (79% homologous); Lck (79% homologous), Hck (79% homologous) and Lyn (93% homologous).
SourceLck antibody was produced against a chemically synthesized phosphopeptide derived from the region of human Lck that contains tyrosine 192 (based on Swiss Protein database, accession number P06239). The sequence is conserved in mouse.
PurificationLck antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Lck, ii) the phosphorylated peptide corresponding to Src tyrosine 215, and iii) a generic tyrosine phosphorylated peptide to remove antibody that is reactive with phosphotyrosine (irrespective of the sequence). The final product is generated by affinity chromatography using a Lck-derived peptide that is phosphorylated at tyrosine 192.
HostLck (pY192) glyc antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ApplicationFor Western blotting applications, we recommend using the antibody at a 1:1000 starting dilution.
BufferRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
StorageStore at -20˚C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20˚C. For shipment or short-term storage (up to one week), 2 - 8˚C is sufficient.
- Full length untagged recombinant active human Lck protein.]
- Budagian, V., et al. (2003) Signaling through P2X7 receptor in human T cells involves p56(lck), kinases, and transcription factors AP-1 and NF-kappa B. J. Biol. Chem. 278(3):1549-1560.
- Mustelin, T. and K. Tasken (2003) Positive and negative regulation of T-cell activation through kinases and phosphatases. Biochem. J. 371(Pt 1):15-27.
- Goldmann, W.H. (2002) p56(lck) Controls phosphorylation of filamin (ABP-280) and regulates focal adhesion kinase (pp125(FAK)). Cell Biol. Int. 26(6):567-571.
- Gervais, F.G. and A. Veillette (1997) Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J. Biol. Chem. 272(19):12754-12761.]