FormulationRabbit polyclonal immunoglobulin in phosphate buffer, pH 7.4, with 1.0 mg/mL BSA (IgG, protease-free) as a carrier.
Clonality/CloneThis is a polyclonal antibody.
SpecificityHuman Lck. Mouse Lck [pS158] has not been tested.
SourceLck antibody was produced against a chemically synthesized phosphopeptide derived from the region of Lck that contains serine 158 (based on Swiss Protein database, accession number P06239). The sequence is conserved in human and mouse.
PurificationLck antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using (i) a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Lck, and (ii) a generic serine phosphorylated peptide to remove antibody that is reactive with phosphoserine (irrespective of the sequence). The final product is generated by affinity chromatography using a Lck-derived peptide that is phosphorylated at serine 158.
HostLck [pS158] antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ApplicationFor Western blot applications, we recommend using the antibody at 0.1-1.0 μg/mL. At 0.50 μg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed.
BufferRabbit polyclonal immunoglobulin in phosphate buffer, pH 7.4, with 1.0 mg/mL BSA (IgG, protease-free) as a carrier.
StorageStore at -80˚C. Upon initial thawing, apportion into working aliquots and Store at -80˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Full length untagged recombinant human Lck protein.]
- Fujimaki, W., et al. (2001) Functional uncoupling of TCR engagement and Lck activation in anergic human thymic CD4+ T cells. J. Biol. Chem. 276(20):17455-17460.
- Ellery, J.M., et al. (2000) Activation of the interleukin 2 receptor: a possible role for tyrosine phosphatases. Cell Signal 12(6):367-373.
- Gervais, F.G. and A. Veillette (1997) Reconstitution of interactions between protein-tyrosine phosphatase CD45 and tyrosine-protein kinase p56(lck) in nonlymphoid cells. J. Biol. Chem. 272(19):12754-12761.
- Soula, M., et al. (1993) Anti-CD3 and phorbol ester induce distinct phosphorylated sites in the SH2 domain of p56lck. J. Biol. Chem. 268(36):27420-27427.]