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Lano Antibody | catalog# 3001

Background

Lano Antibody: Lano is a member of the LAP (leucine-rich repeats and PDZ) family of proteins that also includes Densin-180, Erbin, and hScribble. The LAP proteins generally contain multiple leucine-rich repeat (LRR) domains which serve to target them to the basolateral membrane of epithelial cells. Lano is unique in that it alone does not possess one or more PDZ (PSD95/DLG/ZO-1) domains as do the other members of the LAP family. However, it can bind to the PDZ domain of Erbin in addition to those of membrane-associated and guanylate kinase (MAGUK) proteins which regulate adhesion and plasticity at cell junctions. It has been suggested that it is through these interaction that these LAP proteins participate in the maintenance of proper embryonic development and integrity of epithelial tissues.

Description
Lano Antibody
Western blot analysis of Lano in PC-3 whole cell lysate with Lano antibody at (A) 1 or (B) 2 μg /ml.
Lano Antibody
Immunohistochemistry of Lano in human colon tissue with Lano antibody at 10 μg/mL.
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Specifications

Additional Names
Lano
Additional Names
Lano Antibody:
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal Lano Antibody.
Purification
Lano Antibody is affinity chromatography purified via peptide column.
Host
Lano antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
Lano antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human Lano.
Application
Lano antibody can be used for detection of Lano by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
Lano Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat. No. 3001P - Lano Peptide
Storage
Lano antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1216 - PC-3 Cell Lysate
  • Cat. No. 1320 - Human Colon Tissue Lysate]
Species Reactivity
H
Protein GI Number
14701834
Protein Accession Number
AAK72246
Reference
  1. Saito H, Santoni M-J, Jaulin-Bastard F, et al. Lano, a novel LAP protein directly connected to MAGUK proteins in epithelial cells. J. Biol. Chem. 2001; 276:32051-5.
  2. Legouis R, Jaulin-Bastard F, Schott S, et al. Basolateral targeting by leucine-rich repeat domains in epithelial cells. EMBO Rep. 2003; 4:1096-102.
  3. Funke L, Dakoji S, Bredt DS. Membrane-Associated Guanylate Kinases Regulate Adhesion and Plasticity at Cell Junctions. Annu. Rev. Biochem. 2005; 74:219-45.
  4. Bilder D and Perrimon N. Localization of apical epithelial determinants by the basolateral PDZ protein Scribble. Nature 2000; 403:676-80.]