ProSci Incorporated

Antibodies For All Your Research

Production and Services

LGI4 Antibody | catalog# 4513

Background

LGI4 Antibody: The leucine-rich, glioma inactivated gene 4 (LGI4) is a member of the LGI family in which LGI1 is the exemplar. The LGI family consists of four of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 has been identified as a candidate tumor suppressor gene for glioma and plays a role in autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Despite its high homology with LGI1 and similar pattern of expression, mutations in LGI4 have not been found to be associated with ADTLE. However, the LGI4 gene is located in a region linked to benign familial infantile convulsions. Further study revealed that a GC-to-AT polymorphism was correlated with childhood absence epilepsy. Other studies showed that decreasing LGI4 expression in cultured cells inhibits myelination, indicating that LGI4 may play a role in neural development.

Description
LGI4 Antibody
Western blot analysis of LGI4 in human brain tissue lysate with LGI4 antibody at (A) 1 and (B) 2 μg/mL.
SIZE
PRICE(Select options)QTY
Credit Card Processing

Specifications

Additional Names
LGI4 Antibody: Leucine-rich, glioma inactivated 4
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
LGI4 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI4.
Specificity
Two isoforms of LGI4 are known to exist; this LGI4 antibody will recognize only the larger form. This LGI4 antibody is predicted to be specific to LGI4 and not recognize other LGI proteins.
Conjugate
None
Isotype
IgG
Clonality/Clone
This is a polyclonal LGI4 Antibody.
Purification
LGI4 Antibody is affinity chromatography purified via peptide column.
Host
LGI4 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
LGI4 antibody can be used for the detection of LGI4 by Western blot at 1 - 2 μg/mL.
Tested Application
E, WB
Buffer
LGI4 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4513P - LGI4 Peptide
Storage
LGI4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1463 - Rat Brain Tissue Lysate]
Species Reactivity
H, M, R
Protein GI Number
21359654
Protein Accession Number
AAM49552
Reference
  1. Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol.2005; 22:2209-16.
  2. Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene1998; 17:2873-81.
  3. Berkovic SF, Izzillo P, McMahon JM, et al. LGI1 mutations in temporal lobe epilepsies. Neurology2004; 62:1115-9.
  4. Gu W, Sander T, Becker T, et al. Genotypic association of exonic LGI4 polymorphism and childhood absence epilepsy. Neurogenetics2004; 5:41-4.]