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LGI1 Antibody | catalog# 4489

Background

LGI1 Antibody: The leucine-rich, glioma inactivated gene 1 (LGI1) was first identified as a candidate tumor suppressor gene for glioma and may play a role in other cancers. LGI1 is a member of a family of highly related proteins containing leucine-rich repeats (LRRs) which are highly similar to other transmembrane signaling molecules and receptors. LGI1 serves as a ligand to ADAM22, a metalloprotease localized at the synapse. Mutations in LGI1 account for nearly half of autodominant lateral temporal epilepsy (ADTLE), an epileptic syndrome characterized by focal seizures with predominant auditory symptoms. Two isoforms of LGI1 are known to exist; this LGI1 antibody will recognize only the longer form.

Description
LGI1 Antibody
Western blot analysis of LGI1 in HeLa cell lysate with LGI1 antibody at 2 μg/mL.
LGI1 Antibody
Immunohistochemical staining of human brain tissue using LGI1 antibody at 2.5 μg/mL.
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Specifications

Additional Names
LGI1 Antibody: Leucine-rich, glioma inactivated 1, epitempin, EPT
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
LGI1 antibody was raised against a 14 amino acid synthetic peptide from near the center of human LGI1.
Specificity
This LGI1 antibody is predicted to be specific to LGI1 and not recognize other LGI proteins.
Conjugate
None
Isotype
IgG
Clonality/Clone
This is a polyclonal LGI1 Antibody.
Purification
LGI1 Antibody is affinity chromatography purified via peptide column.
Host
LGI1 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
LGI1 antibody can be used for the detection of LGI1 by Western blot at 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
LGI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 4489P - LGI1 Peptide
Storage
LGI1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1201 - HepG2 Cell Lysate]
Species Reactivity
H, M, R
Protein GI Number
37182888
Protein Accession Number
AAQ89244
Reference
  1. Chernova OB, Somerville RP and Cowell JK. A novel gene, LGI1, from 10q24 is rearranged and downregulated in malignant brain tumors. Oncogene1998; 17:2873-81.
  2. Fialka F, Gruber RM, Hitt R, et al. CPA6, FMO2, LGI1, SIAT1 and TNC are differentially expressed in early- and late-stage oral squamous cell carcinoma - A pilot study. Oral Oncol.2008;
  3. Gu W, Gibert Y, Wirth T, et al. Using gene-history and expression analysis to assess the involvement of LGI genes in human disorders. Mol. Biol. Evol.2005; 22:2209-16.
  4. Fukata Y, Adesnik H, Iwanaga T, et al. Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. Science2006; 313:1792-5.]