FormulationRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
Clonality/CloneThis is a polyclonal antibody.
SpecificityHuman LAT. Mouse (85% homologous) and rat (77%) LAT have not been tested, but are expected to react.
SourceLAT antibody was produced against a chemically synthesized phosphopeptide derived from a region of human LAT that contains tyrosine 132.
PurificationLAT antibody was purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated LAT. The final product is generated by affinity chromatography using an LAT-derived peptide that is phosphorylated at tyrosine 132.
HostLAT [pY132] antibody was raised in rabbit.
Please use anti-rabbit secondary antibodies.
ApplicationFor Western blotting, we recommend using the antibody at 0.1-1.0 μg/mL. At 0.35 μg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed.
BufferRabbit polyclonal immunoglobulin in Dulbecco's phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), with 1.0 mg/mL BSA (IgG, protease free) as a carrier.
StorageStore at -80˚C. Upon initial thawing, apportion into working aliquots and Store at -80˚C. Avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
- Perez-Villar, J.J., et al. (2002) Phosphorylation of the linker for activation of T-cells by Itk promotes recruitment of Vav. Biochemistry 41(34):10732-10740.
- Sommers, C.L., et al. (2002) A LAT mutation that inhibits T cell development yet induces lymphoproliferation. Science 296(5575):2040-2043.
- Lin, J., and A. Weiss (2001) Identification of the minimal tyrosine residues required for linker for activation of T cell function. J. Biol. Chem. 276(31):29588-29595.
- Zhang, W., et al. (2000) Association of Grb2, Gads, and phospholipase C-gamma 1 with phosphorylated LAT tyrosine residues. Effect of LAT tyrosine mutations on T cell antigen receptor-mediated signaling. J. Biol. Chem. 275(30):23355-23361.]