ProSci Incorporated

Antibodies For All Your Research

Production and Services

KRAS Antibody | catalog# 27-016

Background

KRAS is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma.This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region.

Description
KRAS Antibody
Western blot analysis of KRAS in fetal heart lysate using KRAS antibody at 1 μg/ml (12% gel).
SIZE
PRICE(Select options)QTY
Credit Card Processing

Specifications

Additional Names
KRAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, KI-RAS, KRAS1, KRAS2, NS3, RASK2
Positive Control
  • Cat. No. XBL-10407 - Fetal Heart Tissue Lysate]
Disclaimer
This product is for research use only.
Clonality/Clone
This is a polyclonal antibody.
Purification
Antibody is purified by peptide affinity chromatography method.
Host
KRAS antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human KRAS.
Application
KRAS antibody can be used for detection of KRAS by ELISA at 1:62500. KRAS antibody can be used for detection of KRAS by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Tested Application
E, WB
Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.
Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store KRAS antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Species Reactivity
H, M, R, Ds
Protein Accession Number
NP_203524
Molecular Weight
22 kDa
Reference
  1. Turbiner, J., (er) Mod. Pathol. (2008) In press.]