KGF Recombinant Protein | catalog# 40-161
Background
Keratinocyte Growth Factor (KGF/FGF-7) is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of variety of tissues, by promoting cellular proliferation and differentiation. KGF/FG-7 is a mitogen factor specific for epithelial cells and keratinocytes and signals through FGFR 2b. KGF/FGF-7 plays a role in kidney and lung development, angiogenesis, and wound healing. Recombinant human KGF/FGF-7 is an 18.9 kDa protein consisting of 163 amino acid residues.
Specifications
Additional Names
Keratinocyte Growth Factor, Fibroblast Growth Factor-7, HBGF-7Preparation
The ED50 as Biological activity was determined by the dose - dependent stimulation of thymidine uptake by KGF - responsive BaF3 cells is ? 10 ng/ml, corresponding to a specific activity of ? 1 x 105 units/mg.Location
MCNDMTPEQM ATNVNCSSPE RHTRSYDYME GGDIRVRRLF CRTQWYLRID KRGKVKGTQE MKNNYNIMEI RTVAVGIVAI KGVESEFYLA MNKEGKLYAK KECNEDCNFK ELILENHYNT YASAKWTHNG GEMFVALNQK GIPVRGKKTK KEQKTAHFLP MAITSource
E. coliReactivity
n/aTested Application
n/aStorage
The lyophilized KGF recombinant protein is stable for at least 2 years from date of receipt at -20˚C. Reconstituted KGF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚C. As with any protein, exposing KGF recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature.Species Reactivity
n/aPurity
Greater than 95% by SDS-PAGE gel and HPLC analyses.Endotoxin level is less than 0.1 ng per μg (1EU/μg).