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JMJD7 Antibody | catalog# 5385

Background

JMJD7 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. While several JMJD proteins have been identified as being involved in chromatin regulation, histone demethylation and development, the function of JMJD7 has not been identified. JMJD7 was initially thought to be a novel splice form of the phospholipase PLA2G4B which is located downstream of JMJD7 as most tissues express a read-through transcript of the two genes.

Description
JMJD7 Antibody
Western blot analysis of JMJD7 in 3T3 cell lysate with JMJD7 antibody at 1 μg/mL.
JMJD7 Antibody
Immunohistochemistry of JMJD7 in rat liver tissue with JMJD7 antibody at 2.5 μg/mL.
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Specifications

Additional Names
JMJD7 Antibody: Jumonji domain-containing protein 7, JmjC domain-containing protein 7
User Note
Optimal dilutions for each application to be determined by the researcher.
Isotype
IgG
Immunogen
JMJD7 antibody was raised against an 18 amino acid synthetic peptide from near the carboxy terminus of human JMJD7.
Disclaimer
This product is for research use only.
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Conjugate
None
Clonality/Clone
This is a polyclonal JMJD7 Antibody.
Purification
JMJD7 Antibody is affinity chromatography purified via peptide column.
Host
JMJD7 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
JMJD7 antibody can be used for detection of JMJD7 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
JMJD7 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5385P - JMJD7 Peptide
Storage
JMJD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1282 - 3T3 (NIH) Cell Lysate]
Species Reactivity
H, M, R
Protein GI Number
205783894
Protein Accession Number
P0C870
Reference
  1. Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.
  2. Clissold PM and Ponting CP. JmjC: cupin metalloenzyme-like domains in jumonji, hairless and phospholipase A2beta. Trends Biochem.Sci. 2001; 26:7-9.]