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JMJD6 Antibody | catalog# 5383

Background

JMJD6 Antibody: The jumonji domain-containing protein (JMJD) family is defined by the presence of the JmjC domain that is observed in several diverse species. JMJD6 was initially identified as a membrane protein that participates in phagocytosis but recent studies have shown that it has other functions when it is expressed in the cytosol and nucleus. JMJD6 is thought to play important roles in regulation of development and differentiation as knockdown experiments in mice resulted in neonatal lethality with severe defects in the morphology of numerous organs. JMJD6 also can catalyze the lysyl-hydroxylation of U2AF65, a protein involved with RNA splicing, suggesting that some of the functions attributed to JMJD6 may be due to its regulatory activity of RNA splicing.

Description
JMJD6 Antibody
Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 μg/mL.
JMJD6 Antibody
Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 μg/mL.
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Specifications

Additional Names
JMJD6 Antibody: Jumonji domain-containing protein 6, phosphatidylserine receptor, PTDSR, PSR
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.
Immunogen
JMJD6 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human JMJD6.
Isotype
IgG
Clonality/Clone
This is a polyclonal JMJD6 Antibody.
Conjugate
None
Formulation
Physical State: Liquid

Concentration: 1 mg/mL
Purification
JMJD6 Antibody is affinity chromatography purified via peptide column.
Host
JMJD6 antibody was raised in rabbit.

Please use anti-rabbit secondary antibodies.
Application
JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Tested Application
E, WB, IHC, IF
Buffer
JMJD6 Antibody is supplied in PBS containing 0.02% sodium azide.
Blocking Peptide
Cat.No. 5383P - JMJD6 Peptide
Storage
JMJD6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Positive Control
  • Cat. No. 1303 - Human Brain Tissue Lysate]
Species Reactivity
H, M, R
Protein GI Number
67461014
Protein Accession Number
Q6NYC1
Reference
  1. Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.
  2. Fadok VA, Bratton DL, Rose DM, et al. A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature2000; 405:85-90.
  3. Zakharova L, Dadsetan S, and Fomina AF. Endogenous JMJD6 gene product is expressed at the cell surface and regulates phagocytosis in immature monocyte-like activated THP-1 cells. J. Cell. Phys.2009; 221:84-91.
  4. Bose J, Gruber AD, Helming L, et al. The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal. J. Biol.2004; 3:15.]