IHC Service

ProSci, Inc. offers cost effective immunohistochemistry service (IHC service) and immunocytochemistry service (ICC service) for pharmaceutical, biotechnology and academic research institutes employing different methods of detection (fluorescence, alkaline phosphatase and peroxidase) on cells, paraffin and frozen tissue sections. Sections or arrays for IHC service can be provided by either the client or ProSci. Assays are routinely run by highly trained scientists with more than 15 years of experience in immunochemistry assay development and microscopic cell/tissue imaging analysis. Clients can use protocols developed by ProSci or propose their own methods for IHC Service. In addition to IHC service and ICC service, a HE staining service is also available upon request. As a routine matter, complex cases are reported with annotated digitally acquired image, incorporated into the histopathology report.

Typical IHC Service Includes:

• Routine histological preparation:
  • Tissue processing from fixation, embedding, sectioning (serial or single) and H & E staining. Central to this service is proper specimen acquisition in coordination with the tissue retrieval service, recording, reporting and storage.
• IHC staining for novel antibody screening:
  • Optimization of antigen retrieval conditions: using detergent, enzymatic or microwave retrieval methods to expose the target antigen to the antibody.
  • Titration of the primary antibody concentration and adjusting the experimental conditions of the IHC staining.
  • IHC detection with counter staining in tissue and tissue microarrays.
  • Multiplexed detection by dual IHC assays (double immunolabeling).
  • Panel and individual antibody service choices.
  • Highly sensitive colorimetric or fluorescent end points: immunofluorescence, immunoperoxidase or immuno-gold/silver techniques.
• Result analysis and report: technical and full case interpretation.
• Micropohotography: provide publishable images

If you have any questions regarding our IHC service please contact us at abservices@prosci-inc.com, or by calling toll-free 888.513.9525.