Human Normal Tissue Blot IV

Human Normal Tissue Blot IV | catalog# 1524

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are bladder, breast, cervix, kidney, ovary, placenta, prostate, testis, and uterus.

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Specifications

Kit Contents

Lanes: 1, protein markers; 2, bladder; 3, breast; 4, cervix; 5, kidney; 6, ovary; 7, placenta; 8, prostate; 9, testis; and 10, uterus

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.