Human Normal Tissue Blot III

Human Normal Tissue Blot III | catalog# 1523

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are colon, duodenum, esophagus, ileum, small intestine, jejunum, pancreas, rectum, and stomach.

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Specifications

Kit Contents

Lanes: 1, protein markers; 2, colon; 3, duodenum; 4, esophagus; 5, ileum; 6, small intestine; 7, jejunum; 8, pancreas; 9, rectum; and 10, stomach.

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.