Human Normal Tissue Blot II

Human Normal Tissue Blot II | catalog# 1522

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 9 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, heart, kidney, liver, lung, pancreas, skeletal muscle, skin, and spleen.

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Specifications

Kit Contents

Lanes: 1, protein markers; 2, brain; 3, heart; 4, kidney; 5, liver; 6, lung; 7, pancreas; 8, skeletal muscle; 9, skin; and 10, spleen.

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.