Human Normal Tissue Blot I

Human Normal Tissue Blot I | catalog# 1521

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 8 different human normal tissue lysates with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The human normal tissues included in the blot are brain, colon, heart, kidney, liver, lung, pancreas, and spleen.

Description

Coomassie blue staining of human normal tissue lysates in 12% SDS-PAGE. Lanes: 1, protein markers; 2, brain; 3, colon; 4, heart; 5, kidney; 6, liver; 7, lung; 8, pancreas; and 9, spleen.

Credit Card Processing

Specifications

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.