Human Lung Tumor Blot VI

Human Lung Tumor Blot VI | catalog# 1546

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 1 normal human lung tissue, the cell line A549 (human lung carcinoma), and 7 different human lung tumor tissue lysates with 15 μg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20% gradient SDS-PAGE gel and transferred onto nitrocellulose membranes. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, 1 differentiated adenocarcinoma, 1 squamous cell carcinoma, 1 pleomorphic carcinoma and 2 non-diagnosed carcinomas (from different donors).

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Specifications

Kit Contents

Lanes: 1, protein markers; 2, A549; 3, normal lung tissue; 4, small cell carcinoma; 5, non-small cell carcinoma; 6, differentiated adenocarcinoma; 7, squamous cell carcinoma; 8, pleo-morphic carcinoma; 9, non-diagnosed carcinoma, 10, non-diagnosed carcinoma.

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.