Human Lung Tumor Blot IV

Human Lung Tumor Blot IV | catalog# 1513

Background

The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries the cell line A549 (human lung carcinoma), and 3 different human lung tumor tissue lysates and matched normal lysates (from non-tumor lung tissue from near the tumor) with 15 μg of total cellular protein in each lane. Proteins were separated in 4-20% SDS-PAGE gel and transferred onto nitrocellulose membrane. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 differentiated carcinoma, and 1 pleomorphic carcinoma.

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Specifications

Kit Contents

Lanes: 1, protein markers; 2, A549; 3, small cell carcinoma; 4, matched normal lung tissue; 5, differentiated carcinoma; 6, matched normal lung tissue; 7, pleomorphic carcinoma; and 8, matched normal lung tissue.

Application

For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.