Human immunoblots from ProSci are made using human cell lines or human tissues. ProSci human immunoblots are designed for quick and easy protein expression screening of multiple human cell lines or human tissues. Each human immunoblot contains nine lanes, including a pre-stained protein marker and each lane is loaded with 15 μg of total protein. However, our immunoblots are highly customizable. Up to 50 μg of protein can be run on virtually any percentage or gradient percentage gel. Human immunoblot membranes can have more than 9 lanes loaded in any order. Our human immunoblots are usually transferred to nitrocellulose, but PVDF membranes may also be requested.
ProSci human immunoblots can be used with any standard membrane procedure. Our human immunoblots can also be reused after stripping off any assay antibodies from the membrane.