Human ImmunoBlots
Human immunoblots from ProSci are made using human cell lines or human tissues. ProSci human immunoblots are designed for quick and easy protein expression screening of multiple human cell lines or human tissues. Each human immunoblot contains nine lanes, including a pre-stained protein marker and each lane is loaded with 15 μg of total protein. However, our immunoblots are highly customizable. Up to 50 μg of protein can be run on virtually any percentage or gradient percentage gel. Human immunoblot membranes can have more than 9 lanes loaded in any order. Our human immunoblots are usually transferred to nitrocellulose, but PVDF membranes may also be requested.
ProSci human immunoblots can be used with any standard membrane procedure. Our human immunoblots can also be reused after stripping off any assay antibodies from the membrane.
| Product Name | Catalog # | App. | Reactivity | Description |
|---|---|---|---|---|
| Human Cell Line I Blot | 1501 | WB | n/a | MCF HeLa HT-29, 293, Hep G2, A-549, SK-N-SH, PC-3 |
| Human Cell Line II Blot | 1502 | WB | n/a | Daudi, Raji, K-562, U-937, THP-1, HL-60, Jurkat, M |
| Human Lung Tumor Blot II | 1511 | WB | n/a | A549, small, non-small, squamous cell carcinoma, m |
| Human Lung Tumor Blot IV | 1513 | WB | n/a | A549, small cell, differentianted carcinoma, pleom |
| Human Lung Tumor Blot V | 1514 | WB | n/a | A549, small cell, differentianted carcinoma, pleom |
| Human Lung Tumor Blot VI | 1546 | WB | n/a | A549, normal lung tissue, small cell carcinoma, no |
| Human Normal Tissue Blot I | 1521 | WB | n/a | brain, colon, heart, kidney, liver, lung, pancreas |
| Human Normal Tissue Blot II | 1522 | WB | n/a | brain, heart, kidney, liver, lung, pancreas, skele |
| Human Normal Tissue Blot III | 1523 | WB | n/a | colon, duodenum, esophagus, ileum, small intestine |
| Human Normal Tissue Blot IV | 1524 | WB | n/a | bladder, breast, cervix, kidney, ovary, placenta, |