HELB Antibody | catalog# 28-506
Background
The protein encoded by this gene is a DNA helicase. A dominant-negative mutant of this protein blocks chromosomal DNA replication and suggests that its function is required for S phase entry.
Description
Western blot analysis of HELB in Jurkat Cell Lysate using HELB antibody at 2.5 μg/ml (8% gel).
Specifications
Additional Names
HELBDisclaimer
This product is for research use only.Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
HELB antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HELB.Application
HELB antibody can be used for detection of HELB by ELISA at 1:62500. HELB antibody can be used for detection of HELB by western blot at 2.5 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HELB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
HProtein Accession Number
NP_387467Molecular Weight
120 kDaReference
- Taneja, P., et al., (2002) J. Biol. Chem. 277 (43), 40853-40861.]