HDAC6 Antibody | catalog# 27-349
Background
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Description
Western blot analysis of HDAC6 in 293T Cell Lysate using HDAC6 antibody at 1 μg/ml (15% gel).
Specifications
Additional Names
HDAC6Disclaimer
This product is for research use only.Positive Control
- 293T Cell Lysate]
Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
HDAC6 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HDAC6.Application
HDAC6 antibody can be used for detection of HDAC6 by ELISA at 1:62500. HDAC6 antibody can be used for detection of HDAC6 by western blot at 1.0 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HDAC6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, DProtein Accession Number
AAH05872Molecular Weight
16 kDa, 131 kDaReference
- Int J Mol Med. 2003 Jul;12(1):87-93..]