HCLS1 Antibody | catalog# 27-647
Background
HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.
Description
Specifications
Additional Names
HCLS1Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
HCLS1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCLS1.Application
HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:2500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCLS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, RProtein Accession Number
NP_005326Molecular Weight
54 kDaReference
- Ruzzene, M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47.]