HCLS1 Antibody | catalog# 27-445
Background
HCLS1 Tyr phosphorylation catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets. This gene is a substrate for caspase cleavage during apoptosis.
Description
Western blot analysis of HCLS1 in Jurkat Cell Lysate using HCLS1 antibody at 1.25 μg/ml (12% gel).
Specifications
Additional Names
HCLS1Disclaimer
This product is for research use only.Positive Control
- Cat. No. 1205 - Jurkat Cell Lysate]
Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
HCLS1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCLS1.Application
HCLS1 antibody can be used for detection of HCLS1 by ELISA at 1:312500. HCLS1 antibody can be used for detection of HCLS1 by western blot at 1.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WB, IHCBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCLS1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
HProtein Accession Number
NP_005326Molecular Weight
54 kDaReference
- Ruzzene, M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47.]