HCFC1 Antibody | catalog# 28-904
Background
HCFC1 is a member of the host cell factor family with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection.
Description
Western blot analysis of HCFC1 in HepG2 Cell Lysate using HCFC1 antibody at 1.25 μg/ml (12% gel). Marker in lane A.
Specifications
Additional Names
HCFC1Positive Control
- Cat. No. 1211 - HepG2 Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by protein A chromatography method.Host
HCFC1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HCFC1.Application
HCFC1 antibody can be used for detection of HCFC1 by ELISA at 1:312500. HCFC1 antibody can be used for detection of HCFC1 by western blot at 1.25 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HCFC1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, M, R, DProtein Accession Number
AAH63435Molecular Weight
45 kDa, 209 kDaReference
- Director MGC Project, (2003) Direct Submission.]