HBP1 Antibody | catalog# 25-467
Background
HBP1 is a transcriptional repressor that binds to the promoter region of target genes. This protein plays a role in the regulation of the cell cycle and of the Wnt pathway. It binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter, HBP1 is enhanced by interaction with RB1. The protein also can disrupt the interaction between DNA and TCF4.
Description
Western blot analysis of HBP1 in Hela cell lysate using HBP1 antibody at 1 μg/ml (12% gel).
Specifications
Additional Names
HBP1, FLJ16340Positive Control
- Cat. No. 1201 - HepG2 Cell Lysate]
Disclaimer
This product is for research use only.Clonality/Clone
This is a polyclonal antibody.Purification
Antibody is purified by peptide affinity chromatography method.Host
HBP1 antibody was raised in rabbit.Please use anti-rabbit secondary antibodies.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human HBP1.Application
HBP1 antibody can be used for detection of HBP1 by ELISA at 1:1562500. HBP1 antibody can be used for detection of HBP1 by western blot at 1 μg/ml, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.Tested Application
E, WBBuffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μl of distilled water. Final antibody concentration is 1 mg/ml.Storage
For short periods of storage (days) store at 4˚C. For longer periods of storage, store HBP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.Species Reactivity
H, M, RProtein Accession Number
NP_036389Molecular Weight
58 kDaReference
- Paulson, K.E., (2007) Cancer Res. 67 (13), 6136-6145.]